Journals / KBB-Forum / 2021 / Cilt: 20 - Sayı: 1
EVALUATION OF THE PRESENCE OF CALPROTECTIN IN MIDDLE EAR EFFUSIONS FROM CHILDREN WITH OTITIS MEDIA WITH EFFUSION AND ITS RELATIONSHIP WITH TUMOR NECROSIS FACTOR-ALPHA AND INTERLEUKIN-8
- Journal
- KBB-Forum
- Pages
- 45–53
- DOI
- —
Abstract
Objective: The aim of this study is to assess the presence of calprotectin in middle ear effusions (MEEs) in patients with otitis media with effusion (OME) and to determine its relation to proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor-alpha TNF-α in effusions, as well as clinical and epidemiological data. Materials and Methods: Thirty-three children (aged 2.5 to 14 years) diagnosed with chronic OME and scheduled for ventilation tube insertion were included in the study. A total of 41 MEEs and 33 plasma samples were collected during surgery and later, calprotectin, IL-8, and TNF-α levels in the samples were assessed by using commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kits. Total protein levels in MEEs were measured by bicinchoninic acid (BCA) protein Assay. Results: Calprotectin, IL-8, and TNF-α were detected in 100%, 95.1%, and 43.9% of effusions, respectively. Mean levels of calprotectin, IL-8, and TNF-α determined in effusions were 581.4 ± 740 ng/mg total protein, 3379.2 ± 4329.6 pg/mg total protein, and 53.6 ± 69.7 pg/mg total protein, respectively. Mean calprotectin concentration in the plasma samples was 123.11 ± 59.40 ng/ml. A positive correlation was detected between measured values of calprotectin and TNF-α (p < 0.05). There was no correlation between the measured values of calprotectin, IL-8 or TNF-α and patient age or duration of effusion. Conclusion: Calprotectin seems to participate in the inflammatory process during OME. However, further studies are needed to reveal the full extent of calprotectin's role in OME which may help to develop novel treatment strategies.
Özet
Amaç: Bu çalışmanın amacı efüzyonlu otitis media (EOM) olan hastalarda, orta kulak efüzyonlarında kalprotektin varlığını değerlendirmek ve kalprotektinin efüzyonlardaki proenflamatuar sitokinler olan interlökin-8 (IL-8) ve tümör nekroz faktör-α (TNF-α), klinik ve epidemiyolojik verilerle ilişkisini belirlemektir. Yöntem ve Gereçler: Kronik EOM tanısı alan ve ventilasyon tüpü yerleştirilmesi planlanan 33 çocuk hasta (2.5-14 yaşlar arası) çalışmaya dahil edildi. Ameliyat sırasında toplam 41 orta kulak efüzyonu ve 33 plazma örneği toplandı ve daha sonra numunelerdeki kalprotektin, IL-8 ve TNF-α seviyeleri, piyasada bulunan sandviç enzime bağlı immünosorban test (ELISA) kitleri kullanılarak değerlendirildi. Efüzyon örneklerindeki toplam protein seviyeleri bisinkoninik asit (BCA) protein testi ile ölçüldü. Bulgular: Kalprotektin, IL-8 ve TNF-α, efüzyonların sırasıyla %100, %95.1 ve %43.9'unda saptandı. Efüzyonlarda saptanan ortalama kalprotektin, IL-8 ve TNF-α seviyeleri sırasıyla 581.4 ± 740 ng / mg total protein, 3379.2 ± 4329.6 pg / mg total protein ve 53.6 ± 69.7 pg / mg total protein idi. Plazma örneklerindeki ortalama kalprotektin konsantrasyonu 123.11 ± 59.40 ng/ml idi. Ölçülen kalprotektin ve TNF-α değerleri arasında pozitif korelasyon saptandı (p