Journals / Journal of the Turkish Chemical Society, Section A: Chemistry / 2017 / Cilt: 4 - Sayı: 3
Evaluation of Total Polyphenol Content, Antioxidant Activity and Chemical Composition of Methanolic Extract from Allium Kharputense Freyn et. Sint. and Determination of Mineral and Trace Elements
- Pages
- 691–708
- DOI
- —
Abstract
Allium kharputense Freyn Et. Sint. (A. kharputense) was extensively investigated for analyzing total polyphenol content, antioxidant activity, chemical composition and mineral and trace element composition. Total polyphenolic content was determined as 257 mg GA 100 g-1 dried weight by using Folin-Ciocalteu method. DPPH test was performed for antioxidant activity analysis. IC50 values of gallic acid and Trolox were determined as 0.02642 mg mL-1 and 0.225 mg mL-1, respectively. IC50 value of A. kharputense was found as 2.186 mg mL-1. DPPH free radicalscavenging activity of extract from 1 mg of A. kharputense was determined as 0.01207 mg GAE and 0.1029 mg TrE, respectively. Chemical composition was determined using GC-MS. 28 compounds were detected in methanolic extracts of A. kharputense and these compounds were evaluated based on their medicinal and pharmaceutical effects. 20 minerals and trace elements were determined along with their levels by ICP-MS using microwave digestion procedure for preparing acid extracts