Preparation and Cytotoxicity of Coriandrum sativum L. Oil-Loaded Chitosan Nanoparticles

Abstract

Coriandrum sativum L. (coriander) oil has antibacterial, antifungal, and antimicrobialactivity. This study prepared and characterized chitosan nanoparticles loaded with the oil fromCoriandrum sativum L., using an ionic gelation method with tripolyphosphate as the cross-linker.The coriander oil-loaded chitosan nanoparticles were characterized by Zeta Sizer measurements,Fourier transform infrared spectrometry, scanning electron microscopy (SEM), electrondispersive X-ray spectroscopy (EDS), and in vitro release properties. It was shown that chitosannanoparticles loaded with coriander oil have an average size of 113.5 nm, zeta potential of 16mV and a polydispersity index of 0.378. The encapsulation efficiency was calculated as 72%.The spherical morphology of the nanoparticles was verified by SEM analysis, and EDS revealedthe elemental composition. Furthermore, cytotoxicity analysis using L929 fibroblast cells and theXTT method demonstrated that coriander oil-loaded chitosan nanoparticles, coriander oil, andempty chitosan nanoparticles had no significant cell toxicity.