Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes

Abstract

In the present study, the extract of an endemic plant ourkey Marrubium sivasense Aytaç,Akgül & Ekici was investigated for phytochemical profile, antioxidant properties and inhibition ofseveral enzymes. The chemical composition of the extract was investigated with LC-MS/MStechnique. Forsythoside B, verbascoside and leucoseptoside were identified as the main compounds.The free radical scavenging activity of the extract was determined against DPPH• radicals (IC50 0.34mg/mL) and ABTS•+ cation radicals (TEAC 2.3 mM). In the xanthine-xanthine oxidase (XO) systemand in β-carotene bleaching assay the extract demonstrated moderate activity (Inh% 16.54 andInh% 43±1.26, respectively). The extract demonstrated hypoglycemic activity (Inh% 49.67±1.09)via inhibition of porcine pancreatic α-amylase. The antiinflammatory effect tested via inhibition of 5-LOX was found as Inh% 18.71±0.74. However, no antityrosinase activity was found.