Voltammetric Analysis of Cephalexin and Cefazolin in Pharmaceutical Formulations and Biological Samples

Abstract

This study presents the use of disposable pencil graphite electrode to investigate theelectrochemical behaviors and voltammetric determination of cephalexin and cefazolin in phosphate bufferat different pH values (4.5-6.0). Sample analysis was performed in phosphate buffer at the optimummedium determined for each sample. Validation parameters were studied to show the correctness,sensitivity, and consistency of the method developed for the cephalosporins. It was concluded thatdisposable pencil graphite electrode could be used effectively in the determination of cephalosporins. Thelimits of detection (LOD) (S/N=3) were found to be 0.117 mM and 0.293 mM, for cephalexin and cefazolin,respectively. The analysis of these compounds in pharmaceutical formulations and in human blood serumand urine samples was carried out at defined optimum conditions.