Can Soybean Lipoxygenases be Real Models for Mammalian Lipoxygenases? A Bioinformatics Approach

Abstract

Soybean lipoxygenase-1 is one of well-studied enzymes because it is considered as a modelenzyme for mammalian lipoxygenases. In general, the soybean lipoxygenase-1 is used in the test ofinhibitory activities of various compounds. The present study provides a bioinformatics approach forcomparison of various lipoxygenases in the databases. Their various physical and chemical parameterssuch as molecular weight, theoretical pI, amino acid composition, aliphatic index, and grand average ofhydropathicity and the multiple sequence alignments of the lipoxygenases were computed by usingseveral bioinformatics tools. In order to see phylogenetic relationships among lipoxygenases, aphylogenetic tree was constructed. The first three most abundant amino acids in soybean lipoxygenase-1and 15-lipoxygenase (human) are L (10.3 %), S (7.4 %), A (6.7 %) and L (13.3 %), G (7.4 %), V (7.1 %),respectively. According to the phylogenetic tree, the soybean lipoxygenases are within separate cladecompared to the mammalian lipoxygenases. In conclusion, soybean lipoxygenase-1 may not fullycharacterize the human lipoxygenase-15 since there are remarkable sequence-based differences, whichare obtained by using bioinformatics tools between soybean lipoxygenase-1 and lipoxygenases from othersources especially from human. In this context, the researchers, who are aware of the problems describedabove and who have similar concerns, have begun to use recombinant DNA technology to producerecombinant human lipoxygenase-15 enzyme.Keywords: Bioinformatics, lipoxygenases, soybean