INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT TRACHEA SMOOTH MUSCLE CONTRACTIONRELAXATION RESPONSE

Abstract

Ammonium pyrrolidine dithiocarbamate (APDTC) is a suppressive thiol compound of Nuclear Factorkappa B (NF-κB) and works to prevent infections, regulate oxidation, prevent cell death, affects onviruses. Therefore, it is recommended to avoid harmful practices and to give importance to activesubstance research in medical ethics. In postoperative care, respiratory system relaxation treatmentsare important. There is insufficient research into how the functions of APDTC are regulated in humanand animals. In our study, we aimed to evaluate the function of APDTC in rat trachea smooth muscle.70 male Wistar albino rats were used. The rats were euthanized by giving anesthesia and afterapplying cervical dislocation the trachea was separated, connective tissues of trachea were separatedand placed in Krebs solution. Potassium chloride (KCl), Acetylcholine (ACh), APDTC, Atropine,Phentolamine, Propranolol, Nifedipine and Tetraethylammonium (TEA) were added in to the solution.Eventually, APDTC produced a relaxation response in the tracheal smooth muscle induced by ACh,but this relaxation was not statistically significant. In our study, Half maximal effective concentration(EC50) dose was found to be ineffective. APDTC did not induce L-type Ca2+ channel and K+channelreceptors to differentiate relaxation response by producing a cholinergic-adrenergic effect. We thinkthat APDTC did not affect these channels. Further studies with the use of different doses arerecommended.