Detection Of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Abstract

This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance ofanimal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats anddogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefiximetellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detectthe resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole andtetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samplesfrom cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least onevirulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%)were susceptible to all antimicrobials.