Cefaclor monohydrate loaded microemulsion formulation for topical application: Characterization with new developed UPLC method and stability study

Abstract

The purpose of this study was to formulate Cefaclor monohydrate (CEF) loaded microemulsionformulations with the help of pseudo-ternary phase diagrams for topical application. Additionally, in this study also anew ultra-performance liquid chromatography (UPLC) method was developed for the determination of CEF, whichwas not previously entered into the literature. The droplet size, polydispersity index, pH, rheology, drug content, FTIR,dissolution study and release kinetic study have been used in the characterization of microemulsion. The UPLCmethod developed was validated for linearity, specificity, precision, sensitivity, accuracy, range and robustness.Linearity was determined to be at a concentration range of 5-55 μg.mL-1. The method developed was decided to beprecise due to RSD values of