Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak & Ekici

Abstract

In this present study, the aerial parts of Verbascum eskisehirensis were dried in the shade and air-flowedarea. The dried samples were macerated with 70% methanol in water for 24 h by three times. The biological activitiesof the extract was determined by in vitro methods. The antioxidant activity was evaluated in 2,2’-diphenyl-1-picrylhydrazyl (DPPH˙) radical scavenging assay and the trolox equivalent antioxidant capacity (TEAC) was measuredthe ability of the extract to scavenge the stable radical cation ABTS+ (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonicacid)). The antibacterial effects against some clinical pathogens were tested by broth microdilution assay. The in vitroantiinflammatory activity was also examined by 5-Lipoxygenase (5-LOX) enzyme inhibitory test. In the same time, thephenolic composition of the extract was determined by LC-ESI-MS/MS system. According to results, the extract wasfound rich-in luteolin derivatives. The major compound was identified as luteolin-pentosyl-hexoside. The antioxidantactivities of the extract were moderate. The extract showed weak antibacterial activity against tested bacteria strains.Salmonella typhimurium ATCC 13311 was more sensitive to the extract than the others. The extract showed weakantiinflammatory activity.