In vitro anticancer property of Solanum mammosum callus culture against HeLa and Vero cell lines

Abstract

Breast cancer and cervical cancer were ranked first and fourth of the leading cause of death of womenin the world. One of natural compounds that was reported on having cytotoxic activity against various cancer cellswith high selectivity is betulinic acid. In our previous study, it was found that the acetone extract of Solanummammosum callus culture contains betulinic acid. The aims of the current study were firstly to investigate theanticancer activity of the acetone extract of S. mammosum as well as betulinic acid, and secondly to quantitativelydetermine the betulinic acid content in the acetone extract of S. mammosum culture. The anticancer activity was carriedout using the MTT method against HeLa and Vero cells. The determination of betulinic acid content was done usingthe TLC densitometry method. The results showed that the acetone extract of S. mammosum gave anticancer activityagainst HeLa cancer cells with IC50 value of 120.5 µg/mL, however, it was less active compared to betulinic acid withIC50 of 19.3 µg/mL. Both samples were found to be non-toxic on Vero normal cells. Based on TLC densitometry, theacetone extract of S. mammosum contain 1.2% w/w betulinic acid.