THE INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT AORTA SMOOTH MUSCLE CONTRACTIONRELAXATION RESPONSES

Abstract

Ammonium Pyrrolidine Dithiocarbamate (APDTC) is an inhibitor of nuclear factor kappa b (NF-κB).Beside of these function, its antitumoral, antioxidant, anticarcinogenic and antiviral properties andalso apoptosis inhibiting effect in smooth muscle cells were determined. Our experiments aimed toinvestigate the mechanism of action of APDTC on rat aortic smooth muscle. Some adrenergic andcholinergic receptors, l-type Ca2+ channels and K+channels were blocked in 7 different groups, andtherefore the action mechanism of APDTC whether is used or not on which channels and receptorsand the extent to which they are effective were aimed to be determined. The contraction-relaxationresponses after the administration of APDTC, atropine, phentolamine, propranolol, nifedipine,tetraethylammonium (TEA) and mix (atropine+phentolamine+propranolol) on living state controlledwith potassium chloride (KCl) and blocked nitric oxide (NO) synthesis with l-ng-nitro arginine methylester (L-NAME) in the pre-contraction-induced aortic preparations with phenylephrine wereinvestigated. The obtained data were evaluated by kruskal wallis and mann-whitney u tests. APDTCcreated the relaxation response in the aortic smooth muscle. Cholinergic receptor blocker atropine, αadrenergic receptor blocker fentolamine, β-adrenergic receptor blocker propranolol, l-type calciumchannel blocker nifedipine and potassium channel blocker TEA did not alter the relaxation response ofAPDTC. In the mix group consisting of atropine+phentolamine+propranolol, APDTC created asignificant contraction response. It has been determined that APDTC can be effective on thesesystems via different mechanisms.