THE INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT BLADDER SMOOTH MUSCLE CONTRACTIONRELAXATION RESPONSES

Abstract

Ammonium pyrrolidine dithiocarbamate (PDTC), the specific inhibitor of NF-κB, is a thiol compoundwith anti-viral, anti-apoptotic, anti-inflammatory and antioxidant effects proven in many studies.There is not sufficient literature in what fashion that APDTC has produced these effects. We aimed toinvestigate the action mechanism of APDTC in rat bladder smooth muscle in our vitro study. In ourstudy, 70 Wistar albino male rats were used. The rats were euthanized by cervical dislocation underthe anesthesia and then abdomens were opened and bladders were isolated. After the removal of theconnective tissues around the bladders, they were placed in the organ bath containing Krebs solution.KCl, ACh, APDTC, Atropine, Phentolamine, Propranolol, Nifedipine and TEA were applied to thebath with an appropriate protocol. The obtained data were evaluated with Kruskal Wallis and MannWhitney U tests. ACh induced bladder produced APDTC relaxation response in its own smoothmuscle. Cholinergic receptor blocker Atropine, β-adrenergic receptor blocker Propranolol, L-typecalcium channel blocker Nifedipine and atropine+phentolamine+propranolol mix have not changedthe relaxation response that APDTC produced. In the presence of potassium channel blocker TEA,APDTC produced contraction response, but this was not a significantly important grade response. Inthe presence of α-adrenergic receptor blocker Phentolamine, APDTC produced significant contractileresponse. As a result, we think that APDTC shows its effect on rat bladder smooth muscle via αadrenergic receptors and NANK system.