The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion

Abstract

Objectives: The effects of ozone treatment on direct mechanical injury and the inflammation secondary to the injury resulting from bluntthoracic trauma were evaluated with the histopathological results obtained in our study.Patients and Methods: 24 adult male Wistar albino rats of the same colony with an average weight of 200-250 gr were used in our study.The rats were divided into 3 groups. First group were anaesthetized with ketamine HCl/xylazine. Second group were given contusion withisolated unilateral lung contusion. Third group were given 30 µgr/ml ozone at a dose of 1.1mg/kg intrarectally after the contusion for five days.Four different parameters; atelectasis, parenchymal inflammation, perivascular mononuclear inflammation and bronchial injury, were used inhistopathological evaluation and all parameters were scaled.Results: Cell concentration, perivascular mononuclear inflammation and leukocyte infiltration were significantly higher in the lung tissues of allthe rats in the trauma group compared to the anesthesia group. However, when the control, trauma and ozone groups were compared, therewas no significant difference in terms of alveolar edema and bronchial injury. Similarly, among subgroubs of trauma and ozone groups, therewas not a statistical significance in terms of histopathological data.Conclusion: When inflammatory stages and cell distribution were compared between the study groups, advanced acute inflammatory changeswere found on the fifth day of inflammation in both contusion groups and no significant difference was observed in terms of inflammatoryresponse pattern. Consequently, no positive effect of ozone treatment was shown on the acute and subacute inflammatory response indecreasing the primary and secondary injury caused by the trauma.