Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Abstract

Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malignphenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bonemarrow-derived mesenchymal stem cells (BM-MSCs) to have this ability due to cell-cell interaction, released cytokines, and organization withthe extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character.Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in3-dimensional (3D) in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3Dstructure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects. The invasion andmigration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independentsamples. All P-values