Journals / JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES / 2020 / Cilt: 5 - Sayı: 2
Antioxidant Activity and Total Phenolic and Flavonoid Contents of Salvia verticillata L., Salvia tomentosa Mill., and Phlomis lychnitis L.
- Pages
- 125–130
- DOI
- —
Abstract
The purpose of our study was to investigate the total phenolic, total flavonoid, andantioxidant properties of methanol and ethyl acetate extracts of Salvia verticillata L., S. tomentosaMill., and Phlomis lychnitis L. The antioxidant potentials of the extracts were determined using(2,2-diphenyl-1-picrylhydrazyl) DPPH free-radical scavenging activity. The total phenoliccontent (TPC) and total flavonoid content (TFC) were evaluated using the spectrophotometricmethod. The methanol extract of S. verticillata was the highest in TPC and TFC. The same extractrevealed the most significant antioxidant effect with SC50 value of 0.01±0.mg/mL. Strong positivecorrelations were also found between DPPH and TFC (r = 0.904; p < 0.01), and TPC (r = 0.963;p < 0.01). This relationship indicated that flavonoids, together with other phenolic compounds,contribute greatly to this bioactivity. Our results further suggest that the methanol extract of S.verticillata L. is a high-quality antioxidant for use in the medical and food industries.
Özet
Çalışmamızın amacı, Salvia verticillata L., S. tomentosa Mill. ve Phlomis lychnitis L.'nin metanol ve etil asetat ekstraklarının toplam fenolik, toplam flavonoid ve antioksidan özelliklerini araştırmaktır. Ekstraktların antioksidan potansiyelleri DPPH (2,2-difenil-1-pikrilhidrazil) serbest radikal temizleme etkinliği kullanılarak belirlendi. Toplam fenolik içerik (TPC) ve toplam flavonoid içerik (TFC) spektrofotometrik yöntem kullanılarak değerlendirildi. S. verticillata’nın metanol ekstraktı, TPC ve TFC’de en yüksektir. Aynı ekstrakt, 0.01 ± 0.00 mg / mL SC50 değeri ile en iyi antioksidan aktivite ortaya koydu. Ayrıca DPPH ve TFC (r = 0.904; p