Paraoxonase Activity and Oxidized LDL Levels as Cardiovascular Risk Factors in Patients with Coronary Artery Disease

Koroner Arter Hastalarında Kardiyovasküler Risk Faktörü Olarak Paraoksonaz Aktivitesi ve Okside LDL Düzeyleri

Ahmet BELCE, Hakan EKMEKÇİ, Hafize UZUN, Raif Umut AYOĞLU, Özlem Balcı EKMEKÇİ, Özgür Bilgin AYOĞLU

Abstract

Objective: The aim of this study was to investigate the possible relationship between serum oxidized low-density lipoprotein (Ox-LDL) concentration, recently shown to be a cardiovascular risk factor, paraoxonase 1 (PON1) activity and Thiobarbituric Acid Reactive Substance (TBARS) levels, and the severity of Coronary Artery Disease (CAD) as determined by the obstructive vessel number inpatients. Methods: We determined plasma PON1 activity, serum Ox-LDL concentration, Apolipoprotein A1 (ApoA1) levels, TBARS levels and lipid profiles, including total cholesterol (TC), triglycerides (TG), Low-Density Lipoprotein cholesterol (LDL-C), High-Density Lipoprotein cholesterol (HDL-C), and Very Low-Density Lipoprotein cholesterol (VLDL-C), in patients with CAD (n=42) and in a healthy control group (n=17). The CAD group was divided into three subgroups with single- (n=13), double- (n=15) and triple-vessel (n=14) disease according to their angiography results. PON1 activity and TBARS levels were measured spectrophotometrically; Ox-LDL levels were determined by ELISA; ApoA1levels were determined immunoturbidometrically; and TC, TG, and HDL-C levels were determined enzymatically.Results: In the study we observed that PON1 activity decreases when the number of affected vessels increases (r=-0,537, p

Özet

Amaç: Bu çalışmada koroner arter hastalarında damar tıkanıklığının şiddetine bağlı olarak serum Okside LDL (Ox-LDL) konsantrasyonu; son zamanlarda kardiyovasküler risk faktör olarak tanımlanan Paraoksonaz 1 (PON1) aktivitesi ve tiobarbitürik asit reaktif substansları (TBARS) düzeyleri arasındaki olası ilişki araştırıldı. Yöntemler: Okside LDL, Apolipoprotein AI (Apo AI), TBARS düzeyleri, PON1 aktivitesi lipid profili (Total Kolesterol (TK), Trigliserid (TG), LDL Kolesterol (LDL-K), HDL Kolesterol (HDL-K), VLDL Kolesterol (VLDL-K) düzeyleri 42 koroner arter hastası ve 17 sağlıklı bireyde belirlendi. Hasta grubu anjiografi sonuçlarına göre tek damar (n:13), iki damar (n:15) ve üç damar (n:14) olarak 3 alt gruba bölündü. PONI aktivitesi ve TBARS düzeyleri spektrofotometrik, Ox-LDL düzeyleri Eliza, Apo-AI immunoturbidimetrik, TK, TG ve HDL-K düzeyleri ise enzimatik yöntemlerle tayin edildi. Bulgular: Çalışmamızda damar tıkanıklık derecesi arttıkça PON1 aktivitesinin azaldığı gözlendi (r: -0,537, p