Pharmacologically induced absence seizures versus kindling in Wistar rats

Abstract

OBJECTIVE: This study aimed to investigate the effects of γ-butyrolactone (GBL), a prodrug of gamma-Hydroxybutyric acid-induced absence seizures on the development of kindling in Wistar rats.METHODS: Three groups of adult male Wistar rats under anesthesia were implanted with bilateral cortical recording electrodes for the GBL group (GBL) and/or bipolar stimulation electrodes into the right basolateral amygdala for the Kindlinggroup (KI) alone and Kindling plus GBL group (GBL+KI). Rats in the KI and GBL+KI groups were stimulated twice daily atthe afterdischarge threshold until they reached Racine’s stage 5 seizure state. The animals in the GBL + group had an i.pinjection of GBL 20 minutes before each electrical stimulation, and the effects of GBL-induced seizures on the developmentof kindling were investigated. The animals in the GBL group were injected GBL twice daily i.p. for 15 days without receivingany electrical stimulation.RESULTS: The KI animals reached stage 5 seizure stage at 12th stimulations, whereas the GBL+KI rats reached at 27thstimulations. The mean numbers of stimulations needed for the development of the first stage 3, 4, or 5 generalized seizureswere significantly higher in the GBL+KI group than the KI group.CONCLUSION: The resistance to amygdala kindling in the GBL model can be modulated by the absence seizure mechanismalone, without the intervention of an abnormal genetic background.