Alkol tüketimi, ilaç kullanımı ve bazı hastalıklarda eritrosit katalaz aktiviteleri

Erythrocyte catalase activities in alcohol consumption, medications and some diseases

Abstract

Catalase (CAT) has a catalytic role in the decomposition of hydrogen peroxide (H2O2) and a peroxidic role in which the peroxide is utilized to oxidize a range of H donors. The objective of the present study was to determine whether alcohol consumption, medications, and diseases have an effect in the erythrocyte on CAT activity or not. For this purpose, the subjects were divided into three different groups with their unique criteria: 1-alcoholic and nonalcoholic, 2- subjects under medication and no medication, and 3- subjects with leukemia, hepatitis, diabetes mellitus, and heart diseases. CAT activity was measured by the method of Aebi. The hemoglobin levels were determined by Olympus AU-600 auto analyzer. Hematological parameters such as MCH HCT, MCHC, MCV, and RBC were studied by Coulter STKS instrument. There was no effect of medication on CAT activity 3155$pm$1039 k/g Hb for subjects under medication and 3051$pm$956 for other subjects (p>0.05). When CAT activities of the disease groups with leukemic cardiac, hepatitis, and diabetic were compared to the control group, there were no significant differences between them. We found that enzyme activity was affected significantly by only alcohol consumption. CAT activities were 3059$pm$958 k/g Hb in nonalcoholic subjects and 3644±984 k/g Hb in alcoholic subjects (p

Özet

Katalaz (CAT) hidrojen peroksidin parçalanmasında katalitik rol oynarken, peroksidin hidrojen donörüne okside edilmesinde peroksidik bir rol oynar. Bu çalışmada alkol tüketimi, ilaçlar ve hastalıkların eritrosit katalaz aktivitesi üzerine etkisini araştırmak amaçlanmıştır. Bu amaç için denekler 3 farklı gruba ayrıldılar; 1- alkol alan ve almayan 2-ilaç alan ve almayan 3- lösemi, hepatit, diabetes mellitus ve kardiyak hastalıkları olanlar. Katalaz aktivitesi Aebi metodu ile ölçüldü. Hemoglobin düzeyleri Olympus AU-600 otoanalizörüyle tayin edildi. MCH, HCT, MCHC, MCV ve RBC gibi hematolojik değerler Coulter STKS cihazıyla çalışıldı. İlaç alan ve almayanların katalaz aktivitesinde bir farklılık gözlenmedi (3155 ± 1039 k/g Hb ve 3051 ± 956 k/g Hb, p>.0,05). Sağlıklı kişilerdeki ile lösemililerin, kalp hastalarının, hepatitlilerin ve diabetes mellitusluların katalaz aktiviteleri arasında fark bulunamadı. Alkol ile enzim aktivitesi belirgin bir şekilde arttı. CAT aktiviteleri alkol almayanlarda 3059 ± 958 k/g Hb ve alkol alanlarda 3644 ± 984 k/g Hb olarak bulundu (p