In vitro antifungal susceptibility of yeasts causing vulvovaginitis

Süleyha HİLMİOĞLU, Macit İLKİT, Işıkgöz Meltem TAŞBAKAN, Cengiz ÇAVUŞOĞLU

Abstract

This study aimed at determining the in vitro antifungal susceptibility of agents of vulvovaginal candidosis to topical antifungals used in topical treatment. In vitro antifungal susceptibility of 106 Candida strains isolated as causative agents of vulvovaginal candidosis was investigated using Fungitotal. The tested antifungals were miconazole, econazole, ketoconazole, tioconazole and clotrimazole. Candida albicans was the most common isolated organism (42.5%), followed by C. glabrata (41.5%), C. tropicalis (9.4%), C. krusei (4.7%) and C. kefyr (1.9%). Fifty-two (49.1%) of the Candida strains were found susceptible to all antifungal drugs, only one (0.9%) C. glabrata strain was found resistant. These results shows the necessity of antifungal susceptibility test in cases of vulvovaginal candidosis resistant to topical antifungal treatment.

Özet

Bu çalışmada, vulvovaginal kandidozun sağaltımında kullanılan topikal antifungallerin in vitro antifungal duyarlılıklarının belirlenmesi amaçlanmıştır. Vulvovaginal kandidoz etkeni olarak soyutlanan 106 Candida kökeninin in vitro antifungal duyarlılığı Fungitotal kiti ile araştırıldı. Test edilen antifungaller; mikonazol, ekonazol, ketonazol, tiyokonazol ve klotrimazol idi. En sık soyutlanan tür Candida albicans (%42.5) olup bunu C. glabrata (%41.5), C. tropicalis (%9.4), C. krusei (%4.7) ve C. kefyr (%1.9) izledi. Test uygulanan Candida kökenlerinden 52'si (%49.1) tüm antifungal ilaçlara duyarlı, bir (%0.9) C. glabrata kökeni ise dirençli bulundu. Bu sonuçlar, topikal antifungal sağaltıma dirençli vulvovaginal kandidoz olgularında antifungal duyarlılık testinin uygulanmasının gerekliliğini göstermektedir.