Cytokine activity in the plasma of patients with Hepatitis C infection

Hepatit C virus infeksiyonu bulunan hastaların plazmasında bazı sitokinlerin aktiviteleri

Saim YOLOĞLU, Bay Aysun KARABULUT, Çetin ÖZTÜRK, Yaşar BAYINDIR, Engin GÖZÜKARA, Emine SÖNMEZ

Abstract

Some cytokines [interleukin (IL) - 1, IL-2, IL-6, IL-8, IL-10, IL-12, Tumor Necrosis Factor alfa (TNF - alfa)] are effective on liver function of healthy and sick individuals. The purpose of this study was to determine cytokine activity in patients with Hepatitis C Virus (HCV) infection. The levels of IL - 1$beta$, IL 2R, IL 6, IL 8 and TNF-alfa in patients with hepatitis C were determined with chemiluminance method. Ten healthy volunteers were selected as controls (Group I), 10 patients with acute hepatitis C (Group II), 10 patients with chronic hepatitis C (Group III), and 10 patients with chronic hepatitis C who received six months of INF therapy (9 million U/week) (Group IV). In Groups I, III, and IV; IL-1$beta$ was on undetectable level. In Group II, IL- 1$beta$ was 9.82 ±0.11 pg/ml, and there was significant difference between group II and the others. IL-2R levels in Groups I, II, and IV were between 515.9 ± 23.35 and 563.05 ± 5.22 U/ml. In Group III, IL-2R was 775.85 ± 7.98 U/ml. In Groups l, II, III; IL-6 < 5 pg/ml; in Group IV IL-6 was 6.84 ± 0.22 pg/ml. IL-6 was 5.05 pg/ml in Group IV. TNF-alfa was 5.75 ± 0.36 pg/ml in Group I, and in groups II, III, IV it was between 10.49± 0.5 and 16.76 $pm$ 0.12 pg/ml. As a result, IL-1$beta$ was found higher in acute HCV infection, IL-2R and IL-8 were higher in patients with untreated chronic HCV infection. IL-6 was higher in chronic HCV patients treated with Interferon. TNF-alfa was found higher in all groups except in the control group. Futures studies can enlighten the effect of cytokines on chronic hepatitis C infection and its therapy.

Özet

Sitokinlerden interlökin (IL) -1, IL-2, IL-6 IL-8, IL-10, IL-12, Tümör Nekrozis Faktör alfa (TNF-alfa)'nın hem sağlıklı hem de hasta bireylerde karaciğer fonksiyonları üzerine etkisi vardır. Amacı, Hepatit C Virüs (HCV) infeksiyonunda bazı sitokinlerin aktivitlerini saptamak olan bu çalışmada, HCV'li olgularda IL-1$beta$, IL-2R, IL-6, IL-8, ve TNF-alfa düzeyi kemiluminesans yöntemiyle ölçüldü. Bu amaçla Turgut Özal Tıp Merkezi'ne gelen 10 sağlıklı, gönüllü birey kontrol olarak (Grup l), akut hepatit C'li 10 hasta (Grup II), tedavi görmemiş kronik hepatit C'li 10 hasta (Grup III) ve 6 aylık interferon tedavisini ( 3x3 milyon Ü/hafta) tamamlamış kronik hepatit C'li 10 hasta (Grup IV) olmak üzere toplam 40 kişi çalışmaya alındı. Grup I, III, IV 'de IL-1$beta$