Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD

Abstract

In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysisof lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the floweringand fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columnswith diatomaceous earth (Extrelut®). The chromatographic separation was achieved employing an isocratic system witha mobile phase including trifluoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diodearray detector (DAD). The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.