Determination of sultamicillin in bulk and pharmaceutical preparations via derivatization with NBD-Cl by spectrophotometry

Abstract

Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceuticalpreparations has been developed.Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform.Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range forsultamicillin was 10-50 µg.$mL^{-1}$ with a correlation coefficient of 0.9999. The limit of detection (LOD) and limit of quantification(LOQ) were found to be 1.47 and 4.41 µg/$mL^{-1}$, respectively.Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsforindustrial analysis.