Journals / International Journal of Chemistry and Technology (IJCT) / 2020 / Cilt: 4 - Sayı: 2
Quantitative analysis of phenolic compounds of commercial basil cultivars (Ocimum basilicum L.) by LC-TOF-MS and their antioxidant effects
- Pages
- 179–184
- DOI
- —
Abstract
Basil (Ocimum basilicum L.), an aromatic and medicinal plant, is used for food and pharmaceutical purposes. In this work, quantitative analyses of phenolic compounds for commercial basil cultivars, which are Sweet (1), Purple (2), Lettuce (3), Brosh (4) grown in Tokat city in Turkey's ecology, were executed by Liquid Chromatography Time-of-Flight Mass Spectrometry (LC-TOF-MS). Antioxidant activities of related genotypes were determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH)’s radical, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)’s diammonium salt and the ferric reducing antioxidant power (FRAP) assays. The activity-compound relationship was revealed. Brosh (4) genotype revealed the most DPPH [296 µmole TE (Trolox equivalent/g DW (gram dry weight)], ABTS (706 µmole TE/g DW), and FRAP (650 µmole TE/g DW) activities. It was determined that rosmarinic acid was in the highest amount in all genotypes. Among the genotypes, it was determined that Lettuce contained the most rosmarinic acid with a value of 180460.6 (mg kg-1 DW).
Özet
Aromatik ve tıbbi bir bitki olan reyhan (Ocimum basilicum L.) gıda ve ilaç amaçlı kullanılmaktadır. Bu çalışmada, Türkiye'nin ekolojisinde Tokat şehrinde yetişen Sweet (1), Purple (2), Lettuce (3), Brush (4) olmak üzere dört ticari fesleğen çeşidinin fenolik bileşiklerinin kantitatif analizleri LC-TOF/MS ile gerçekleştirilmiştir. İlgili genotiplerin antioksidan aktiviteleri, 2,2-Difenil-1-pikrilhidrazil (DPPH) radikali, 2,2-azino-bis(3-etilbenzotiazolin-6-sülfonik asit (ABTS)’in diamonyum tuzu ve indirgenme gücü (FRAP) yöntemleri kullanılarak belirlendi. Aktivite-bileşik ilişkisi gösterildi. Brush 4 genotipi en yüksek DPPH [296 µmol TE (Trolox ekivalent) /g DW (gram kuru ağırlık)], ABTS (706 µmol TE/g DW) ve FRAP (650 µmol TE/g DW) aktivitesi gösterdi. Rosmarinik asidin bütün genotiplerde en yüksek miktarda olduğu belirlendi. Genotipler arasında Lettuce (3)’ün, 180460.6’ lik (mg kg-1 kuru bitki) bir değer ile en fazla rosmarinik asit içerdiği belirlendi.