In vitro shoot regeneration of oregano (origanum minutiflorum o.schwarz & davis)

Abstract

In vitro shoot regeneration procedure for oregano (O. minutiflorum O. Schwarz & Davis), endemic species of Turkey, is described. Leaf segments and shoot explants (hypocotyl, single nodal segment and shoot tip) excised from 30-40 d old in vitro germinated seedlings and cultured on Murashige and Skoog (MS) and Gamborg (B5) media containing different combinations of benzyl amino purine (BAP) (0.0, 1.0, 2.0 or 3.0 mg L-1) and naphtalene acetic acid (NAA) (0.0, 0.1 or 0.5 mg L-1). The single nodal segments were the most succesful explant in all hormone combinations used. MS medium supplemented with 2.0 mg. L-1 BAP + 0.1 mg L-1 NAA was the most effective medium for shoot formation. However for all explants, B5 medium with all phytohormone combinations had no effective success and therefore were not suitable on in vitro shoot regeneration.