Nitric oxide level of aqueous humour after photorefractive keratectomy in Guinea pigs

Abstract

To investigate the level of nitric oxide (NO) in aqueous humour after excimer laser keratectomy in guinea pigs. Methods: NO levels of the aqueous humour were measured by nitric oxide analyser with chemiluminescence assay in three Groups. Group I served as control, 10 m$mu$ excimer laser corneal photoablation was performed in Group II and 20 m$mu$l corneal photoablation in Group III. Results: NO levels of all groups showed no difference when compared to the control (p>0.05). Conclusion: Excimer laser keratectomy did not change the NO level of aqueous humour in guinea pigs 24 hours following photoablation and this finding could be explained by increased transforming growth factor-$beta$1, (TGF-$beta$1) level of aqueous humour after excimer laser therapy.