Detection of human seminal stains in one minute by modified acid phosphatase test

Abstract

Background: The detection of semen is often an important issue in the investigation of rape and other sexual assaults. The most commonly used procedures for semen identification is acid phosphatase test, which are being used in the Forensic laboratories to solve the sexual assault cases. Objectives: The objective was to determine variability in sensitivity of acid phosphatase in detection of human seminal stains by modified acid phosphatase test. Materials and Methods: Totally, 40 samples of human seminal fluid were collected from male volunteers who were neither be connected in blood relation nor showed any heredity to each other. Serial dilution of seminal fluid, preparation of seminal stain, detection and identification of seminal stains steps were used to analyze, and detection done by modified acid phosphatase test. Results: Modified acid phosphatase test can locate a 1/512 serial diluted seminal stain. Minimum level of acid phosphates enzyme detected in a serial dilution sample of seminal stains was 1/512, 1/256, 1/128, and 1/64. Conclusions: The response may vary from person to person according to the quantity of acid phosphatase enzyme in seminal fluid. Semen could be detected in 1 min on dilution up to 1/512.