Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts

Carla Renata SIPERT, Claudia Bosio MENESES, Alessandra Fonseca GAMBINI, Lucas Tofanello OLIVI, Marcelo Dos SANTOS

Abstract

Objective: This study aimed to investigate the cytotoxic and biomodulatory potential of conventional guttapercha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points onperiodontal ligament (PDL) cells in vitro.Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutionsto evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcriptionfollowed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA)(P