Do Resveratrol and Dehydroepiandrosterone Increase Diminished Ovarian Reserve?

Abstract

Objective: In this study, the aim is to observe changes induced by dehydroepiandrosterone (DHEA) andresveratrol (RES) in diminished ovarian follicles that was induced by 4-vinylcyclohexenediepoxide (VCD).Materials and Methods: Twenty four Wistar albino female rats were divided into 3 groups: control, DHEAand RES. Unilateral oophorectomy was performed in control group to remove the right ovary of 4 rats andthe left ovary of 4 rats. After administration of 160 mg/kg VCD, remaining ovaries were removed. Following the same VCD treatment, in DHEA and RES groups, 60 mg/kg DHEA and 20 mg/kg RES were givenfor 45 days respectively and residual ovaries were removed. Hematoxylin-eosin and TUNEL staining wereperformed. Follicle stimulating hormone (FSH), estradiol (E2) and anti-mullerian hormone (AMH) valueswere measured.Results: In control group, VCD-induced apoptosis in follicles increased the TUNEL-positive cell counts(p