Microbiological quality of emulsified type meat products (Salami-Frankfurter sausage) marketed in Kars

Kars' da satışa sunulan emülsifiye tipi et ürünlerinin mikrobiyolojik kalitesi

Abstract

Seventy Frankfurter type sausage samples including 35 unpackaged and 35 vacuum packaged, and 30 unpackaged salami samples from different retail markets in Kars, were analyzed for their microbiological quality. Tests were performed including the counts of aerob mesophile bacteria, coliforms, Enterobacteriaceae, Enterococci spp., Pseudomonas spp., Staphylococci and Micrococci spp., Clostridium perfringens (C. perfringens), yeast and mould the presence of Salmonella spp. were also analyzed on all the samples examined. Aerob mesophile bacteria, coliforms, Enterobacteriaceae, Enterococci spp., Pseudomonas spp., Staphylococci and Micrococci spp., yeast and mould counts were detected at the counts of < 2.0x $10^2$ cfu/g in all salami and vacuum packaged Frankfurter type sausages. Salmonella spp. were not detected on any of the salami and vacuum packaged Frankfurter type sausages. Three salami samples contained C. perfringens at the level of 102 cfu/g, which is in the limit level (Turkish Standards Institute, TSE 979) and four salami samples had C. perfringens at the count of 103 cfu/g. Of the vacuum packaged Frankfurter type sausage samples, two samples contained C. perfringens at the level of 102 cfu/g, which is in the limit level (TSE 980)" and three samples had 103 cfu/g of C. perfringens. Of the unpackaged Frankfurter type sausages, the mean counts per g for total mesophile bacteria and Pseudomonas spp. were $1.3x10^4$ and $6.0x10^4$ cfu/g, respectively. The mean counts for Enterococci spp., Enterobacteriaceae, coliform, Staphylococci and Micrococci spp., yeast and mould counts were $1.1x10^3, 2.8x10^3, 2.4x10^3, 2.6x10^3$ and 1.9x $10^3$ cfu/g, respectively. Microbial load of the 18 unpackaged Frankfurter type sausage samples were E coli positive. Out of 21 samples, 13 were coagulase (+) Staphylococci spp. and 11 of these were, S. aureus. C. perfringens was at a load ranging from < 2.0x $10^2$ to 1.0x $10^4$ cfu/g, with the most being less than < 2.0x $10^2$ cfu/g. Six Salmonella spp. were isolated and idetified out of 35 unpackaged Frankfurter type sausages.

Özet

Kars'da farklı marketlerde satılan 35 adet paketlenmemiş, 35 adet vakum paketlenmiş toplam 70 adet Frankfurter tip sosis örneği ve 30 adet paketlenmiş salam örneği mikrobiyolojik kaliteleri bakımından analiz edildi. Yapılan analizlerde Aerob mezofil genel canlı, koliform, enterobakteri, enterokok, Pseudomonas spp., stafilokok ve mikrokok, C. perfringens, maya ve küfün düzeyi saptandı. Ayrıca tüm örneklerde Salmonella spp.nin varlığı araştırıldı. Bütün salam ve vakum paketlenmiş Frankfurter tipi sosislerde Aerob mezofil genel canlı, koliform, enterobakteri, enterokok, Pseudomonas spp., stafilokok ve mikrokok, maya ve küf sayısı < 2.0x $10^2$ kob/g olarak saptandı. Salam ve vakum paketlenmiş Frankfurter tipi sosislerin hiç birinden Salmonella spp. saptanamadı. C. perfringens, 3 salam örneğinde sınır seviyesi olan 102 kob/g düzeyinde saptanırken (TSE 979), 4 salam örneğinde 103 kob/g düzeyinde saptandı. Vakum paketlenmiş Frankfurter tip sosilerde ise C. perfringens 2 örnekte sınır seviyesi olan 102 kob/g düzeyinde saptanırken (TSE 980), 3 örnekte 103 kob/g düzeyinde saptandı. Paketlenmemiş Frankfurter sosislerde ise ortalama olarak Aerob mezofil genel canlı ve Pseudomonas spp. sırasıyla $1.3x10^4$ ve $6.0x10^4$ kob/g olarak saptandı. Enterokok, enterobakteriler, koliform, stafilokok ve mikrokok, maya ve küf düzeyleri ortalama değerleri sırasıyla, $1.1x10^3, 2.8x 10^3, 2.4x10^3, 2.6x 10^3$ ve $1.9x10^3$ kob/g olarak saptandı. Paketlenmemiş 18 Frankfurter tipi sosis'den E. coli saptandı. 21 örneğin 13'ünden koagulaz (+) stafilokok izole edildi, bunların 11'i S. aureus olarak identifiye edildi. C. perfringens düzeyinin çoğunlukla