Plasma lipid peroxidation levels and erythrocyte glucose-6-phosphate dehydrogenase activity in patients with insulin-dependent diabetes mellitus

İnsüline bağımlı diabetes mellituslu hastalarda plazma lipid peroksit seviyeleri ve eritrosit glukoz-6-fosfat dehidrogenaz aktivitesi

Abstract

Purpose: In this study, plasma malondialdehyde (MDA, an index of lipid peroxidation) and erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity were investigated in 30 patients with insulin-dependent diabetes mellitus and in 20 healthy controls in order to determine whether there is any relation between these parameters and the disease. Material and methods: Blood glucose, plasma MDA levels and Ğ-6-PD activities were measured in 30 patients with insulin dependent diabetes mellitus and in 20 healthy controls. Plasma MDA levels were determined spectrophotometrically according to a method modified from Satoh and Yagi. G-6-PD activities were measured by the method of Beutler which is based on reduction of NADP to NADPH. Results: In the control group, the plasma MDA level (mean ± SD) was found to be 1.92±0.33 nmol/ml, whereas in diabetic patients with neuropathy and retinopathy and in those without any complications, it was 2.97±0.47 nmol/ml and 2.39±0.32 nmol/ml, respectively. Erythrocyte G-6-PD level in the control group was 4.72±1.47 U/gHb, while in the diabetic group with neuropathy and retinopathy and in the diabetic group without any complications it was 2.98±1.59 U/gHb and 3.04±2.49 U/gHb, respectively. Serum glucose level and plasma MDA level in the diabetic group were significantly higher than those of the control group. Erythrocyte G-6-PD levels were found to be significantly lower when compared with the control group (p

Özet

Amaç:Bu çalışmada, insüline bağımlı diabetes mellituslu hastalarda lipid peroksidasyonunun göstergesi olan malondialdehit (MDA) düzeyleri ile ve eritrosit glukoz-6-fosfat dehidrogenaz (G-6-PD) aktivitesi ölçülerek hastalıkla olan ilişkileri araştırıldı. Gereç ve yöntem: Çalışmada 30 insüline bağımlı diabetes mellituslu hasta ile 20 sağlıklı kişiden oluşan kontrol grubunda kan glukozu, MDA düzeyleri ile eritrosit G-6-PD aktivitesi ölçüldü. Plazma MDA tayini, Satoh ve Yagi'den modifiye edilen bir yöntemle, spektrofotometrik olarak yapıldı. Eritrosit G-6-PD aktivitesi Beutler'in NADP'nin NADPH'a indirgenmesi esasına dayanan kinetik yöntemiyle tayin edildi. Bulgular: Sağlıklı kontrol grubunda plazma MDA düzeyleri (ortalama ± SD) 1.92±0.33 nmol/ml bulunurken, komplikasyonsuz diabette 2.39±0.32 nmol/ml, nöropati+retinopatili diabette 2.97±0.47 nmol/ml bulundu. Eritrosit G-6-PD aktiviteleri kontrol grubunda 4.72±1.47 U/gHb, komplikasyonsuz diabetik grupta 3.04±2.49 U/gHb, nöropati+retinopatili diabetik grupta ise 2.98±1.59 U/gHb olarak belirlendi. Serum glukoz düzeylerinde diabetik gruplarda anlamlı bir artış tespit edildi. Diabetik grupların plazma MDA düzeyleri, kontrol grubundan anlamlı derecede yüksek, eritrosit G-6-PD aktiviteleri ise düşük olarak saptandı (p