Development of analytical method for sensitive determination of streptozotocin based on solid phase extraction

Abstract

This study is focused on developing a sensitive analytical method for Streptozotocin (STZ)molecules which is a naturally occurring alkylating antineoplastic agent. It is also knownparticularly toxic to the insulin-producing beta cells of the pancreas in mammals. With thisstudy, a separation-enrichment method based on solid phase extraction was developed for lowlevels of STZ. The synthetized material consist of a multi-walled carbon nanotube/CuO/Cu2Owas used as an adsorbent in column type SPE. Experimental variables such as pH, ionicstrength, desorption solvent, and other parameters were studied and optimized step by step.Analytical merits were obtained under the optimized conditions: linear range (100.00-1500.00ng mL-1), limit of detection (28.57 ng mL-1), relative standard deviation (RSD) % for 250 ngmL-1 of STZ (< 4.2 %, N:3), and pre-concentration factor (125). In order to test accuracy ofthe method, recovery tests were applied successively by using model solutions.