Effects of EGFR inhibitor AG 1478 on MDA-MB-231 and MCF-7 breast cancer cells

Abstract

In this current study, antiproliferative effect of EGFR inhibitor AG1478 was investigated in human breast cancer cell lines. MDA-MB-231 and MCF-7 cell lines were used respectively as triple negative breast cancer and Luminal A breast cancer model. To this end cell viability, cell index values by xCELLigence Real Time Cell Analysis DP instrument and mitotic index analysis were used. The results of the current study showed that AG1478 had cytostatic effects on both of cell lines. The IC50 concentration was determined as 50 µM for MDA-MB-231 and 20 µM for MCF-7 cell line. IC50 concentration was used for mitotic index parameter. IC50 concentrations decreased the mitotic index values of both of cell lines. There were significant differences between the control and the experimental groups (p