Long-term antibacterial properties of fluoride-releasing orthodontic bonding adhesives

Abstract

Objectives: The aim of this study was to compare antibacterial properties of 3 different fluoride-releasingorthodontic adhesives during three months by using the direct contact test (DCT). Materials and Methods: The materials tested in the present study were Transbond Plus (3M Unitek, Monrovia,USA), Light Bond (Reliance Ortho Prod. Inc. Itasca, IL, USA), and Kurasper F (Kuraray Medical Inc.,Okayama, Japan). Eight specimens of each material type were prepared for estimation. For the DCT, wells of96-microtitre plates were coated with each of three bonding adhesives. Eight uncoated wells, using identicalinocula size, served as a positive control (bacteria with medium). A Strep tococcus mutans suspension was placedon the surface of each specimen that was held for 1 hour at 37C. Bacterial growth was monitored for 16 hourswith a temperature-controlled microplate spectrophotometer. The kinetics of the growth in each well wasrecorded continuously at 650 nm in every 30 minutes. Three additional measurements were performed on thesetested materials after aging for 1 week, 1 month and 3 months. One-way ANOVA and Tamhane s T2 multiplecomparison tests were applied to the data. The level of significance was set as p