In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.

Abstract

The aim of this experiment is to develop a protocol to establish the in vitro propagation potential of Lythrumsalicaria L (purple loosestrife). Seeds of L. salicaria were germinated and grown in Murashige Skoog (MS)medium for 30 days. At the end of 30 days, shoot tips of L. salicaria were cultured on MS mediumsupplemented with different combinations and concentrations of 6-benzylaminopurine (BAP; 1, 2 and 3mg/L) and 1-naphthaleneacetic acid (NAA; 0, 0.1 and 0.5 mg/L). Regenerated shoots were transferred to MSsupplemented with varying concentrations of different auxins: NAA (0.5 and 1 mg/L) and indole-3-butyricacid (IBA; 0.5 and 1.0 mg/L) for rooting. Among the cytokinins investigated, BAP indicated the best responseto initiate shoot elongation and multiple shoot formation alone. The highest number of shoot per explant(37±3.38) was obtained in MS medium with 1 mg/L BAP. Although, the highest shoot length (3.1 cm ± 0.48)was found on medium with 1 mg/L BAP+0.5 mg/L NAA, there were no statistically differences among alltypes of medium. Percentage of rooting, shoot length and root length were altered significantly with differentconcentrations of NAA and IBA. Thus, shoot length (9.5 cm±0.46), root length (4.1 cm±0.13) and thepercentage of rooting (100%) increased significantly with a concentration of 1 mg/L NAA. It was concludedthat MS medium with 1 mg/L NAA has been superior for root development compared to other hormoneconcentrations.