Sytnhesis of organoboron amide-ester branched derivatives of poly(itaconic anhydride-alt-2-vinyl-1,3-dioxolane) and cancer cells interaction studies

Abstract

New organaboron amide-ester branched derivatives of poly(itaconic anhydridealt-2-vinyl-1,3-dioxolane) are synthesized by amidolysis and grafting reactions,respectively. The structure and composition of the synthesized compoundsare characterized by FTIR-ATR (Fourier Transform-Infrared-Attenuated TotalReflection) and 1H (13C) NMR (Nuclear Magnetic Resonance) spectroscopy.These novel functionalized boron containing biopolymers are interacted withHeLa (human cervix carcinoma cell) cancer cells and L929 Fibroblast (normal)cells. Their anticancer properties are investigated using a combination of differentbiochemical analysis methods. It is found that both the values of the cytotoxicityand necrotic indexes are increased due to organoboron linkage but theseindexes of organoboron amide-ester derivative are visibly decreased becauseof α-Hydroxy-ω- methoxypoly(ethylene oxide) (PEO) biocompatibilization.The results of these studies allow us to utilize PEO branched derivatives ofsynthesized organoboron copolymers (up to 200 µg mL-1) as therapeutic potentialfunctional copolymer drugs in cancer chemotherapy and Boron-Neutron CaptureTherapy (BNCT).