Hsa-miR-217 Inhibits the Proliferation, Migration, and Invasion in Non-small Cell Lung Cancer Cells Via Targeting SIRT1 and P53/KAI1 Signaling

Abstract

Background: Brain metastasis is a major cause of cancer deathin patients with lung cancer. Sirtuin 1 and hsa-miR-217 have beenidentified to mediate the development of non-small cell lung cancer.Aims: To investigate the roles of hsa-miR-217, its target sirtuin 1,and the P53/KAI1 axis in the brain metastasis from non-small celllung cancer.Study Design: Cell culture study.Methods: Human pulmonary adenocarcinoma brain metastasis cellline PC-14/B were incubated and treated with constructed lentiviralplasmids expressing miR-217 and/or sirtuin 1. BEAS-2B cell line wasused as a control. The targeted regulation of miR-217 to sirtuin 1wasexamined using a dual-luciferase reporter assay. Cell proliferation,migration, invasion, and related protein expression were detected toexamine the effect of the miR-217/sirtuin 1 expression on metastasis.Results: PC-14/B cells expressed higher sirtuin 1 and lower P53 andKAI1 compared with BEAS-2B control cells (p