The Effects of Different Sperm Activator Medias on the Velocity and Movement Style Brown Trout (Salmo trutta Linnaeus, 1792) Sperm Cells

Abstract

This study was aimed to determine the effects of Na+, K+ and Ca2+ ion-based differentsperm activator medias (SAM) on the velocity and movement style of Brown trout (Salmotrutta) sperm cells. SAMs were prepared such as SAM1 (9 mM NaCl, 50 mM Glycine and 20mM Tris), SAM2 (103 mM NaCl, 40 mM KCl, 20 mM HEPES), SAM3 (125 mM NaCl, 30 mMTris), SAM4 (60 mM NaHCO3, 50 mM Tris) and SAM5 (125 mM NaCl, 1 mM CaCl2, 30 mMGlycine, 20 mM Tris), respectively. It was kept at the same pH: 9.0 for all activators.According to the study data, the statistically significant increases (P0.05). As a result, unlike other activators, thepresence of the Ca2+ (1 mM) ion in the SAM5 activator has resulted in an increase in thevelocities of sperm cells in brown trout sperm cells.