Evaluation of dynamic thiol-disulfide balance in children with stage 3-5 chronic kidney disease

Abstract

Aim: We aimed to examine dynamic thiol-disulfide balance, an indicator of oxidative stress, in children with stage 3-5 chronic kidneydisease (CKD).Material and Methods: Native thiol (Nt), total thiol (Tt) and disulfide (DS) levels were determined spectrophotometrically in 27 childrenwith CKD (14 non-dialysis, 13 hemodialysis) and 40 healthy controls.Results: Albumin, Nt, Tt and DS levels were markedly lower in CKD patients than in controls. However, the DS/Nt values were notstatistically significant between the two groups. When Nt, Tt, DS and DS/Nt values were adjusted for albumin levels, Tt and Ntlevels were lower in CKD patients than controls. In the CKD group, Nt and Tt levels were positively correlated with the albumin andestimated glomerular filtration rate and negatively with urea and creatinine.Conclusion: According to these results, decreased Nt and Tt levels in patients with CKD stages 3–5 may be evidence of exposure tooxidative stress.