The effect of amniotic membrane wrapping on colorectal anastomosis in rats undergoing pelvic radiotherapy

Abstract

Aim: This study aimed to investigate the effect of amniotic membrane wrapped onto the anastomosis line following colon resectionand primary anastomosis on anastomosis healing in rats undergoing pelvic radiotherapy.Material and Methods: Fifty-six male Wistar albino rats were randomly allocated to four main groups. Group I, left colon resection andprimary anastomosis; Group II, post-anastomotic amniotic membrane; Group III, preoperative radiotherapy; Group IV, preoperativeradiotherapy and amniotic membrane. Radiotherapy was administered under general anesthesia eight and four days prior to surgeryat a total of 20 Gy. In the first operation, all the rats underwent 1 cm left colon resection and primary anastomosis. The animals ineach group were anesthetized to assess the clinical, mechanical, histologic, and biochemical parameters of anastomotic healing onthe third and seventh postoperative days.Results: In the third day groups, anastomosis line bursting was observed in only one subject in those undergoing amniotic membranewrapping(p