Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 1997 / Cilt: 44 - Sayı: 2-3
The effect of lead acetate administration on some haematological values in guinea pigs
- Pages
- 249–258
- DOI
- —
Abstract
The investigation was carried out on 20 male guinea-pigs weighing 300-350 gr. The guinea-pigs were divided into four groups consisting of five animals in each. Three of them were used for treatment and one as control group. Lead acetate was intraperitoneally injected to the first, second and third treatment group as the doses of 0.1 mg/kg, 0.5 mg/kg and 1 mg/kg respectively.'Injections were performed twice a day for six days. On the 15th and 30th day after the last injection, blood was collected by cardiac puncture. Total red blood cell and white blood cell counts, haemoglobin concentrations, haematocrit values were determined. Differential count was made on the smears stained with May Griinwald- Giemsa. Haemoglobin concentrations obtained on the 15thday were 14.1 ± 1.1, 14.2 ±0.7 and 13.4 ± 1.1 g/dl in the first ,second and third treatment group respectively.However, mean haemoglobin concentration was 15.9 g/dl in the control group. The number of total red blood cells in the second dose group declined temporarily on the 15th day. The decrease was found to be statistically significant (p
Özet
Araştırma, 300-350 gr. canlı ağırlığa sahip 20 erkek kobay üzerinde yürütüldü. Kobaylardan, her biri beş hayvandan oluşan ve biri kontrol, üçü deney grubu olmak üzere dört grup oluşturuldu. Kurşun asetat I., II. ve III. deney gruplarına sırası ile, periton içi, 0.1 mg/kg, 0.5 mg/kg ve 1 mg/kg dozlarında verildi. Uygulamalara günde iki kez olmak üzere 6 gün devam edildi. Son enjeksiyondan sonraki 15. ve 30. günlerde kalpten kan alındı. Alınan kanlardan alyuvar ve akyuvar sayısı, hemoglobin ve hematokrit değerleri tespit edildi. May Griinwald-Giemsa ile boyanmış sürme kan prepar atlarında akyuvar formülü yapıldı. Kontrol grubunda, 15. gündeki hemoglobin derişimi; 15,9 g/dl iken I., II. ve III. deney gruplarının derişimleri sırası ile 14.1 ±1.1, 14.2 ±0.7ve 13.4 ±1.1 g/dl olarak belirlendi. Onbeşinci günde, II. deney grubunda alyuvar sayısında görülen azalma istatistiksel olarak anlamlı bulundu (p