Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 1997 / Cilt: 44 - Sayı: 2-3
The research of blood beta carotene,retinol,glucose and cholesterol concentrations in Holstein cows with various fertility problems
- Pages
- 151–157
- DOI
- —
Abstract
The aim of this study was to research the relationship between blood levels of beta-carotene, glucose, cholesterol, retinol and fertility in cows of postpartum 40-90 days which are of various fertility problems. This study has been carried out between the months of September and March and total 50 Holstein cows divided into two groups. Group 1 consisted of 40 cows which have fertility problems and were between 3-8 of ages. The control group consisted of 10 normal cows. The cows in Group 1 were between postpartum 40 and 90 days and as a result of rectal and vaginal examination, they were found to have metritis (n=15), irregular cycles (n=10) and to be in subestrus (n=15). As for the control group, it consisted of normal postpartum cows showing regular cycle. The levels of cholesterol and glucose were determined by using Reflectron (Bohringer, Mannheim) and by kits of the same firm. As to the levels of retinol and beta-carotene they were determined by UV- Specspectro-photometric method . In statistical analysis, t-test method was used. The mean cholesterol, glucose, beta-carotene and retinol levels in Group 1 cows with metritis, or showing subestrus and irregular cycles were found as 190.96±24.37%mg, 66.21±6.26%mg, 103.07±27.76% $mu$ g, 36.35±12.72% $mu$ g, 170.14±14.23%mg, 36.11±4.13%mg, 53.07±17.26% $mu$ g, 46.35±12.72%|^g, 180.16±14.73 %mg, 41.21±5.16%mg, 63.17±17.66%|ig, 48.35±10.22%|0,g respectively. In control group, the mean cholesterol, glucose, beta-caroten and retinol concentrations were respectively 239.55±28.76%mg, 67.0±6.27%mg, 125.45+22.99 % $mu$ g, 48.07±5.97% $mu$ g. The cows showing irregular cycles or subestrus were found to have lower levels of beta-carotene, and glucose compared with metritis or normal ones (P0.001), There was no significant difference between the cholesterol levels of the Group 1. However, the cholesterol concentration found to be lower with metritis and health cows than in the others. As a result, it has been concluded that beta-carotene, glucose, retinol and cholesterol may be role of in various fertility problems.
Özet
Bu çalışmanın amacı, postpartum dönemde fertilite problemli ineklerin kan beta-karotin, retinol, glikoz ve kolesterol düzeylerini araştırmaktır. Araştırma, yaşlan 3-8 arasında değişen, çeşitli fertilite problemi olan 40 ve sağlıklı 10 (Kontrol) olmak üzere toplam 50 baş Holştayn ırkı inek üzerinde Eylül-Mart aylarında gerçekleştirildi. Çalışma grubu postpartum 40-90. günler arasında bulunan, rektal ve vaginal muayene sonucu metritisli (n=15), suböstras (n=15) ve düzensiz siklus (n=10) gösterdiği saptanan ineklerden oluştu. Kontrol grubunu ise postpartum dönemde bulunan ve düzenli siklus gösteren sağlıklı inekler oluşturdu. Kolesterol ve glikoz değerleri Refletron (Bohringer Mannheim) ile aynı firmanın kitleri kullanılarak, retinol ve beta-karotin düzeyleri ise bildirdikleri UV-Spektro-fotometrik yönteme göre be¬lirlendi. İstatistiksel hesaplamalarda t-test'i kullanıldı. Çalışma grubunda bulunan metritisli, suböstrus ve düzensiz siklus gösteren ineklerde kolesterol, glikoz, beta-karotin ve retinol ortalama düzeyleri sırasıyla %190.96±24.37 mg, %66.21±6.26 mg, %103.07+27.76 ug, %36.35±12.72 $mu$ g; %170.14±14.23 mg, %36.11±4.13 mg, %53.07±17.26 $mu$ g, %46.35±12.72 $mu$ g; %180.16±14.73 mg, %41.21±5.16 mg, %63.17±17.66 $mu$ g, %48.35±10.22 $mu$ g olarak bulundu. Kontrol grubunda ise kolesterol, glikoz, beta-karotin ve retinol ortalama değerleri ise sırasıyla %239.55±28.76 mg, %67.0±6.27 mg, % 125.45122.99 ug, %48.07±5.97 $mu$ g saptandı. Düzensiz siklus ve suböstrus gösteren ineklerin, sağlıklı ve metritisli olanlara oranla beta-karotin ile glikoz düzeyleri düşük bulundu (P