The level of erythrocyte arginase activity in brucellosis

Abstract

The arginase, mainly found in liver (L-arginine ureohydrolase EC 3.5.3.1) is the final enzyme of urea cycle which breaks down arginine into ornithine and urea. This study was carried out on 40 patients (29F, 11 M) with brucellosis, a systemic disease that affects different organs and tissues, and 91 healthy sublects (60 F,31 M). in these two group, the level of erythrocyte arginase activity was measured by TDMU method. The hemoglobin concentration of whole blood was measured at Sysmex K-1000 automatic cell counter. The levels of other serum parameters were measured at Beckman Synchron CX5 autoanalyzer (ALT, AST, GGT, LDH activities, BUN, creatinine, Ca and albumin). The levels of eryhrocyte arginase, serum ALT, AST, GGT, LDH activities and, BUN, creatinine were significantly higher in brucellosis as compared with controls. On the other hand , the levels of serum Ca and albumin were significantly lower in brucellosis as compared with controls (p< 0.05, p< 0.01 ). The results of this study indicate that the causes of brucellosis can also make changes in the level of erythrocyte arginase activity as in the other biochemical parameters measured in this study.

Özet

Esas olarak karaciğerde bulunan arginaz (L-arginin üreohidrolaz EC 3.5.3.1) üre döngüsünün son enzimi olup arginini ornitin ve üreye parçalamaktadır. Bu çalışma; sistemik bir hastalık olup birçok organ ve dokuları etkileyen brusella'lı 40 hasta (29 K, 11 E) ile 91 sağlıklı kişide {60 K,31 E) yapılmıştır. Her iki grupta eritrosit arginaz aktivite düzeyi spektrofotometrik TDMU metodu ile ölçülmüştür. Tam kan hemoglobin derişimi Sysmex K-1000 otomatik tam kan sayım cihazında saptanmıştır. Diğer serum parametre düzeyleri (ALT, AST,GGT, LDH aktiviteleri, BUN, kreatinin, kalsiyum ve albumin) ise Beckman Synchron CX5 otoanalizöründe ölçülmüştür. Bruselloziste eritrosit arginaz, serum ALT, AST, GGT, LDH aktiviteleri ve BUN, kreatinin düzeyleri önemli oranda artış gösterirken serum kalsiyum ve albumin düzeyleri kontrollere oranla azalmıştır (p