Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 67 - Sayı: 1
Toxic effects of cutaneous and oral exposure to aluminum and magnesium nanoparticles on brain tissue in rats
- Pages
- 41–49
- DOI
- —
Abstract
In this study, it was aimed to research the effects of cutaneous and oral exposure to aluminum nanoparticles (Al-NPs) and magnesium nanoparticles (Mg-NPs) on the brain tissue, which is vitally important in terms of its structure and functions. The study was performed on Wister-Albino rats, which were divided into 10 groups, such as control groups (groups 1 and 2), groups, to which Al and Mg NPs were applied as 500 mg/kg and 1500 mg/kg orally (groups 3-6) and 1000 mg/kg and 2000 mg/kg cutaneously (groups 7-10). The dosages were administered as a single dose. While brain tissue and serum MDA levels as well as brain tissue TNF-α and IL-6 levels have significantly increased in the group, to which 1500 mg/kg Mg-NPs was applied orally, significant decreases have also been observed in brain tissue GPX and SOD levels of the same group. Additionally, meaningful decreases in brain tissue SOD levels and significant increases in TNF-α and IL-6 levels have been observed in the group, to which 1500 mg/kg Al-NP was applied orally. On the other hand, it was found that brain tissue GPX and SOD levels of the group, to which 2000 mg/kg Mg-NP was applied cutaneous, have been decreased significantly. Histopathological examinations have also supported these findings. At the end of the study, it was observed that the toxic effect of Al and Mg NPs has varied, depending on the application method, dosage and duration.
Özet
Bu çalışmada, alüminyum nanopartiküllerine (Al-NP) ve magnezyum nanopartiküllerine (Mg-NP) önemli maruziyet yollarından olan deri ve ağız yoluyla maruziyetin, yapısı ve görevleri itibarıyla hayati öneme haiz beyin dokusu üzerindeki etkilerinin araştırılması amaçlandı. Çalışma; kontrol grupları (grup 1 ve 2), Al ve Mg NP’lerin 500 mg/kg ve 1500 mg/kg dozlarda ağız yoluyla (grup 3-6) ve 1000 mg/kg ve 2000 mg/kg dozlarda deri yoluyla (grup 7-10) verildiği gruplarda, tek doz uygulamalar yapılarak, 10 grup Wistar-Albino rat üzerinde gerçekleştirildi. Uygulama gruplarından Mg-NP’nin ağız yoluyla 1500 mg/kg dozda uygulandığı grupta beyin dokusu ve serum MDA düzeyleri ile beyin dokusu TNF-α ve IL-6 düzeyleri önemli ölçüde artarken, beyin dokusu GPX ve SOD düzeylerinde önemli azalmalar olduğu gözlendi. Ağız yoluyla Al-NP’nin 1500 mg/kg dozda verildiği grupta beyin dokusu SOD düzeylerinde anlamlı azalmalar ve TNF-α ve IL-6 düzeylerinde ise önemli artışlar tespit edildi. Diğer taraftan, deri yoluyla Mg-NP’nin 2000 mg/kg dozda verildiği grubun beyin dokusu GPX ve SOD düzeylerinde önemli bir azalma olduğu belirlendi. Histopatolojik incelemeler de bulguları destekledi. Çalışma sonucunda, Al ve Mg NP’lerin toksik etkisinin uygulama yoluna, verilen doza ve süreye bağlı olarak değiştiği gözlendi.