In Vitro Evaluation of Gilaburu (Viburnum Opulus L. ) Juice on Different Cell Lines

Abstract

The purpose of this study is to investigate the effects of gilaburu juice on cell viability (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) and angiogenesis (tubeformation assay) using different cell lines (human cancer cell lines A549, Caco-2, HeLa andnormal cell lines MDCK and HUVEC) in vitro. In addition, the genotoxic effects of gilaburu juiceis evaluated using COMET assay on HUVEC cells. Our results demonstrate that gilaburu juicecould inhibit the growth of Caco-2 and HeLa cancer cell lines, but could not significantly inhibitnormal cell lines and A549 cancer cell lines. It disrupted tube formation of HUVEC cells. Gilaburujuice appears to have no genotoxic potential to the DNA of HUVEC cells. The results obtained inthis study confirm the potential application of commercial gilaburu juice as a functional food inprevention of cancer.