The Effect of Delivery Mode on Thiol-Disulfide Balance in the Umbilical Cord Blood

Abstract

Purpose: Today, the Oxidative stress level is frequently determined through the methods examining the thiol-disulfide balance. We aimed to compare oxidative stress in elective caesarean sections (ECS) and vaginal deliveries (VD) by examining the thiol-disulfide balance. Materials and methods: Forty- eight VDs and 57 ECSs were included in the study. For standardization, pregnant women who underwent spinal anesthesia for ECS were examined in the study. Five milliliter of blood samples were taken from the umbilical artery immediately after placenta delivery. Serum native thiol, total thiol, disulfide was analyzed in blood samples. Disulfide/native thiol (%), disulfide/total thiol (%), and native thiol/total thiol (%) ratios were calculated. Results: Native thiol, total thiol, disulfide, and disulfide/total thiol ratios were significantly lower in the ECS group than in the VD group (446.27±48.01 vs 484.26±47.50, p0.05). First and 5th Apgar scores of the newborn of the ECS group were higher than the VD group (p

Özet

Amaç: Günümüzde, oksidatif stress seviyesi sıklıkla thiol-disufid dengesini inceleyen metodlar yoluyla saptanır. Elektif sezaryen (ES) ve vajinal doğumlardaki (VD) oksidatif stresi tihol-disulfid dengesini inceleyerek karşılaştırmayı amaçladık. Araçlar ve Yöntem: Çalışmaya 48 VD ve 57 ES dahil edildi. Çalışmada, standardizasyon amacıyla, spinal anestezi ile yapılan ES’lar incelendi. Plasenta ayrıldıktan hemen sonra umblikal arterden beş mililitre kan örneği alındı. Serum nativ thiol, total thiol, disulfid analiz edildi. Disulfid/nativ thiol (%), disulfid/total thiol (%), and nativ thiol/total thiol (%) oranları hesaplandı. Bulgular: ES grubundaki nativ thiol, total thiol, disulfid, and disulfid/total thiol oranları VD grubundan belirgin olarak düşüktü (446.27±48.01 , 484.26±47.50, p0.05). ES grubundaki yenidoğanların ilk ve 5. dakika Apgar skorları VD grubundan daha yüksekti (p