Karbon tetraklorür hepatotoksisitesine karşı E vitamini etkisinin ve glutatyon, glutatyon peroksidaz değişikliklerinin araştırılması

Investigation of the effect of vitamin E against carbon tetrachlorur hepatotoxicity and alterations of glutathione, glutathione peroxidase

Abstract

The protective effect of vitamin E against carbon tetrach-loride (CC14) hepatotoxicity, glutathione (GSH) and glutathione peroxi¬dase (GSH-Px) alterations were investigated in rats. Lipidperoxidation, which is an indicator of cellular injury in the liver, GSH, GSH-Px and serum enzyme activities (biochemical indicators of hepalotoxicly) were evaluated to this end. Totally, 32 rats were divided into four equal gro¬ups: Control (Group I), CC14 (Group II), vitamin E + CC14 (Group III), and vitamin E (Group IV). A single dose (0.5 ml/kg) of CC14 was injec¬ted intraperitoneally (i.p.) to the rats in group II, Thirty mg of vitamin E (dl-alpha tocopherol) was intraperitoneally administered following a single dose (0.5 ml/kg) ofCCl4 injection to the rats in the group III. Af¬ter 24 hours, blood samples were taken for ALT and AST determinations. Tissue samples were taken to measure lipid peroxidation, GSH and GSH-Px levels. When all groups were compared to the control group, it was found that liver lipid peroxide levels (P<0.001), and serum AST (P<0.001) and ALP (P<0.001) activities were significantly higher in group II and group III. In addition, it was found that liver GSH (P<0.001) levels descreased, while GSH-Px activity was unchanged. No significant alteration was detected in liver lipid peroxide, GSH, GSH-Px and serum transaminases in group IV, compared to the control group (P>0.05). When compared to the carbon tetrachloride group, it was ob¬served that liver lipid peroxide (P<0.02) levels and ALT (P<0.005) le¬vels significantly decreased, while GSH, GSH-Px and AST levels rema¬ined unchanged in group III (P>0.05). In group IV, it was determined that liver lipid peroxide, GSH, AST and ALT activities were significantly lower, (P<0.001), while GSH-Px (P<0.02) activities were higher. We are of the opinion that vitamin E does not significantly affect the known hepatotoxic effect ofCCU, that the protective effect of vitamin E is still controversial, and that further studies are needed on this matter.

Özet

Raflarda E vitamininin karbon tetraklorür (CC14) hepatotoksisi¬tesine karşı etkileri ile glutatyon (GSH) ve glutatyon peroxidaz (GSH-Px) değişiklikleri incelendi. Bu amaçla, karaciğerde hücresel hasarın göstergesi olan lipid peroxidasyonu ile beraber, GSH, GSH-Px ve hepa-totoksisitesinin biyokimyasal göstergeleri olan serum enzim aktiviteleri de değerlendirildi. Toplam 32 rat eşit ayrıldı: Kontrol (Grubu I); CC14 (Grup II), E vitamini+CCl4 (Grup III) ve E vitamini (Grup IV). Grup Wdeki rotlara tek doz (OJ ml/kg) intraperitoneal (i.p.) olarak CC14 en-jekte edildi. Grup Ill'deki ratlara tek doz (0.5 ml/kg) CC14 enjeksiyonun¬dan hemen sonra 30 mg E vitamini (dl-alfa tokoferol asetat) i.p. olarak uygulandı. 24 saat sonra, serum transâminaz (AST, ALT) düzeylerini be¬lirlemek içinde kan örnekleri; lipid peroxidi, GSH ve GSH-Px düzeyleri¬ni ölçmek için doku örnekleri alındı. Bütün gruplar kontrol grubuyla karşılaştırıldığında; Grup II ve III'de karaciğer lipid peroxid düzeyleri (P 0.05). Karbon tetraklorür grubuyla karşılaştırıldığında; Grup III'de, karaciğer lipid peroxid (P 0.05). Grup IVde, karaciğer lipid peroxid, GSH, AST ve ALT aktivitelerinin anlamlı olarak düştüğü (P<0.001), GSH-Px (P<0.02) aktivitelerinin ise yüksek olduğu saptandı. Sonuç olarak, E vitaminin CCl4'ün bilinen hepatotoksik etkisini önemli derecede etkilemediği, E vitamininin protektif etkisinin genel kanının ak¬sine henüz tartışmalı sayılabileceği ve bu konuda daha ileri çalışmalara gereksinim olduğu görüşüne varıldı.