Journals / Turkish Journal of Gastroenterology / 2003 / Cilt: 14 - Sayı: 3
Aktif gastrit ve duodenal ülseri olan Helicobacter pylori-pozitif hastalarda klas I ve II MHC reseptörleri
- Pages
- 168–172
- DOI
- —
Abstract
Background/aims: Helicobacter pylori colonizes on the epithelial surface below the mucous membrane in the gastric antrum, causing chronic active gastritis and duodenal ulcer. However, we know little about the response of the immune system, which is responsible for protecting the organism, to this bacteria and the duodenal tissue damage. The aims of our study were to examine the expression of class I and II MHC receptors in the immune cells in the peripheral venous blood of patients with chronic active gastritis and duodenal ulcer. Methods: The study included a total of 124 cases (Helicobacter pylori-positive patients without prior treatment, 47 of whom had small-sized and 56 medium-sized duodenal ulcer, and 21 healthy individuals). Immune cells carrying receptors CD3, CD4, CDS/4, CDS, CD3/8, CD14, CD45, CD14/45, HLA-DR, CD3/HLA-DR, CD16/66, and CDS 116156 by applying DAKO Dual Color Reagent, and non-specific indicators of reactivity, were investigated in the peripheral venous blood samples. Results: In both study groups, nonspecific indicators of reactivity in peripheral venous blood were not different from those in the healthy group irrespective of the ulcer size. It was detected that NK cells which express only CD3/16/56 receptor are more aggressive than class I MHC carriers, which were increased in both patient groups more markedly than in the control group (p<0.05). The number of CD14 cells in patient groups with small- and medium-sized duodenal ulcers was found to be lower than in the control group. This difference was more marked in the small-sized ulcer group than in the medium-sized ulcer group. While there was a similarity in the number ofCD45 cells in all three groups, the number of CD14/45 cells was reduced in the study group. This reduction was found statistically insignificant in the medium-sized ulcer group, but significant in the small-sized ulcer group (p<0.05). As compared to the control group, the number of HLA-DR cells was found to be increased in both study groups, and CD3/HLA-DR was found to be increased in the medium-sized ulcer group (p<0.05). Conclusions: The reduced number of CD14 cells may have been among the factors predisposing to the development of Helicobacter pylori infection. Such a deficit in cellular defense may be compensated through the upregulation of cells with HLA-DR and CDS IHLA-DR markers by the organism. The increase in the number of cells expressing CD3/16/B6 in chronic antral gastritis and duodenal ulcer patients positive for Helicobacter pylori suggests a role for autoimmune events in these diseases.
Özet
Amaç: Helicobacter Pylori, mide epiteline kolonize olarak kronik aktif gastrit duodenal ülser hastalığına naden olur. Ancak organizlayı koruyan immün sistemin Helicobacter Pylori'ye verdiği yanıt tam olarak bilinmemektedir. Bu çalışmanın amacı Helicobacter Pylori (+) kronik aktif gastritli ve duodenal ülserli hastaların venöz kanındaki immün hücrelerdeki class I ve II MHC reseptör ekspresyonunu araştrmaktır. Yöntem: Çalışmaya daha evvel tedavi görmemiş Helicobacter Pylori (+) 124 olgu alındı (47 küçük çaplı ülser, 56 orta çaplı ülser ve 21 sağlıklı ol-gu). Olguların periterik venöz kan örneklerinde CDS, CD4, CD3/4, CD8, CD3/8, CT>14, CD45, CD14/46, HLA-DR, CD3IHCA-DR, CD16/56 ve CD3/16/56 reseptörleri araştırıldı. Bulgular: CD3/16/56 reseptörlerini sunan NK hücreleri hasta grubunda kontrol grubuna göre daha fazla bulunmuştur. CD14 sunan hücrelerin sayısı hasta grubunda kontrol grubuna göre daha düşük bulunmuştur (p<0.005). Bu bulgu küçük çaplı ülser grubunda daha belirgin idi. CD45 eksprese eden hücrelerin sayısı 3 grupta da benzer iken, CD14/45 eksprese eden hücrelerin sayısı çalışma grubunda azalmıştır. Bu bulgu küçük çaplı ülser grubunda belirgindir (p<0.05). Kontrol grubu ile karşılaştırıldığında HLA-DR hücrelerinin sayısı çalışma grubunda artmıştır, CD3/HLA-DR sayısı ise orta çaplı ülser grubunda artmıştır (p<0.05). Sonuç: CD14 eksprese eden hücrelerin sayısında azalma Helicobakter Pylori infeksiyonu gelişmesine yol açan faktörler arasında olabilir. CD3/16/56 eksprese eden hücrelerin sayısınınHeyicobacter Pylori (+) aktif gastritve ülserli hastalarda artmış olması, bu hastalarda otoimmün olayların rolü düşündürmektedir.