Simultaneous Detection of Flavobacterium psychrophilum, Pseudomonas plecoglossicida, and Vibrio anguillarum by a Multiplex PCR Targeting the gyrB Region

Özet

We have developed a multiplex PCR to detect bacterial disease agents of Flavobacterium psychrophilum, Vibrioanguillarum, and Pseudomonas plecoglossicida. Three primer pairs designed based on determined nucleotide sequences of thegyrB regions of these three bacteria were used. The detection limits and stringency of this method were higher enough forspecific detection of these three fish diseases. Using this multiplex PCR, the rapid and simultaneous diagnosis of three majorbacterial fish diseases caused by these bacteria from the organs of diseased fishes was successful.