Subaraknoid Kanamayı İzleyen Deneysel Serebral Vazosp azmda Proantosiy anidinin Terapötik Değeri

The Therapeutic Value of Proanthocyanidin in Experimental Cerebral Vasospasm Following Subarachnoid Hemorrhage

Ayhan TEKİNER,Muhammet Bahadır YILMAZ,Elif POLAT,Tuncer GÖKER,MUSTAFA FEVZİ SARGON,Anıl ARAT

Abstract

AIM: We aimed to examine the basilar artery in an experimental subarachnoid hemorrhage (SAH) model both from the ultrastructural and radiological aspects and tried to evaluate the benefit of proanthocyanidin (PC), an antioxidant matter, in reducing vasospasm. MATERIAL and METHODS: A total of 21 male New Zealand rabbits were divided into three groups after performing angiography. Group I: Control group, Group II: Subarachnoid hemorrhage group, Group III: Subarachnoid hemorrhage and Proanthocyanidin group (100 mg/kg/ day). Rabbits were sacrificed on the 5th day after angiography. A segment of the basilar artery, 4 mm in length, was prepared for ultrastructural examination. RESULTS: There was no ultrastructural or radiological abnormality in the basilar artery in Group 1. In the basilar artery digital subtraction angiography (DSA) of Group 2, a statistically significant decrease in basilar artery calibration was found (p<0.05). There was no damage in the endothelium, subendothelium, tunica media and adventitia of the basilar artery ultrastructurally but a statistically significant narrowing in lumen diameter was seen. There was also no difference in basilar artery calibration in the DSA of Group 3 (p>0.05). No damage was seen in the basilar artery ultrastructurally. CONCLUSION: Proanthocyanidin with its strong antioxidant effect and possible vasomotor action can significantly attenuate SAH-induced vasospasm.

Özet

AMAÇ: Deneysel subaraknoidal hemoraj i modeli oluşturarak, bir antioksidan olan proantosiyanidinin baziller arterdeki vazospazmı azaltmadaki etkisini hem radyoloj ik hem de ultrastrüktürel olarak incelemek. YÖNTEM ve GEREÇLER: Toplam 21 erkek Yeni Zelanda tavşanı serebral anj iyografi sonrası 3 gruba ayrıldı. Grup I: Kontrol, Grup II: Subaraknoid kanama, Grup III: Subaraknoid kanama ve Proantosiyanidin uygulaması (100 mg/kg/gün). Tavşanlar anj iyografi sonrası 5. günde sakrifiye edildi. 4 mm uzunluğundaki baziller arter segmentleri ultrastrüktürel inceleme için hazırlandı. BULGULAR: Grup Ide baziller arterde ultrastrüktürel ve radyoloj ik anormallik yoktu. Grup IInin baziller arter kalibrasyonunda istatistiksel anlamlı azalma saptandı (p 0,05). Baziller arterde ultrastrüktürel zedelenme görülmedi. SONUÇ: Güçlü antioksidan ve muhtemel vazomotor etkili proantosiyanidin, subaraknoid kanamaya bağlı vazospazmı anlamlı düzeyde azaltabilir.